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「Papa, I'm going to be a star in Heaven.」
「As long as you always come back to me, like Halley's comet.」

The head of the Mewtwo project, Dr. Fuji, only agreed to work for Team Rocket in order to fund his own research. He was already very interested in cloning living beings and reawakening pre-existing consciousness, all in order to regain the daughter he lost in a tragic car accident.

However, unlike the legendary Mew, Ai (Amber)'s genetic makeup is too weak to withstand the process and none of her clones are truly viable. Her consciousness (as well as her physical body in another telling) lives only within the tank, locked in suspended animation, until she passes away after four short years.

Mad with grief, Dr. Fuji recreates her every time. Ai remembers each and every time and can only hope her father would someday stop trying to do the impossible, but she bears through with it, even though she's quite tired of it.

Ever a comforting, motherly soul, she spends her last moments comforting her father and then Mewtwo, thanking him for his tears, but urging him to be happy instead, because he's still alive.

Once Metwo is born, having his traumatic memory of Ai and the other clones' deaths sealed away, he finds out he has neither a papa or a mama, nor was he created by the God Ai spoke of, but instead, he has only arrogant human scientists, who viewed him solely as a scientific experiment. Furious, he destroyed the lab on New Island, killing Dr. Fuji and the rest of the researchers, putting an end to Ai's cycle of death and rebirth.

「They say living beings only cry when their bodies are in pain. Humans are the only ones who let out tears when they’re sad. Thank you.
But please don’t cry, Mewtwo. You’re living! Stay alive, okay? I’m sure it’ll be fun.」